Excellent S100 from Agilent

Afyon Kocatepe University

Overall

Quality of Results

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Company:

Agilent

Product Name:

S100

Catalog Number:

Z0311 (GA504)

Clone Number:

S100 isolated from cow brain.

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It is an excellent antibody for multiple species in immunohistochemistry. We use it for both purpose research and tumor diagnosis (domestic animal tumors) In my lab, it is very specific mostly S100B.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Rat intestine

Primary Incubation

1/1000, RT, 2 hours

Blocking Agent

BSA, 1%

Secondary Incubation

Biotinylated Rabbit IgG, RT, 2 hours

Tertiary Incubation

Streptavidin-peroxidase, RT, 1 hours / AEC chromagen, 20', RT

Detection

ABC-peroxidase method

Results Summary

Some human tissue-specific antibodies do not work in animals. But this antibody is giving excellent result for dog, cat, sheep, cow, rat tissues. Image legend: Severe positivity in Auerbach's plexus in the rat intestine (arrows).

DOI or PMID #

N/A

Additional Notes

Gill's hematoxylin as a background staining (image)

Image Gallery

Summary

The Good

Specific, sharp and working multiple species.

The Bad

Very light background staining.

The Bottom Line

This is my favorite S-100. I use it often and never change it.

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