Antibody From BioLegend Stains CD3 Very Well

Stanford

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 700 Anti-Mouse CD3 Antibody

Catalog Number:

100216

Clone Number:

17A2

We wanted to assess how certain perturbations would affect T cells in the spleen, so we used the antibody to identify CD3+ immune cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen.

Primary Incubation

1:100

Blocking Agent

FcBlock

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytek Aurora

Results Summary

We stained mouse spleen cells with the anti-CD3 antibody and found that it identifies CD3+ T cells very well. The antibody was easy to use and the staining was very clear.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody works great for staining CD3.

The Bad

None to mention.

The Bottom Line

The antibody is great for identifying CD3+ T cells.

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