Specific Dog-1 Antibody for Human Tissues (IHC)

Afyon Kocatepe University

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Quality of Results

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Company:

Thermo Fisher Scientific

Product Name:

DOG-1 Recombinant Rabbit Monoclonal Antibody

Catalog Number:

MA5-14582

Clone Number:

SP31

Info:Supplier Page View Product Specs

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Antibody is rabbit monoclonal, excellent affinity bu low dilution. In the other hand it is very expensive. it stain excellent human cajal cells and gastrointestinal stromal tumor cells.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Human intestinal tissues (image human gist)

Primary Incubation

1/25, RT, 2 hours

Blocking Agent

BSA, 1%

Secondary Incubation

Biotinylated Rabbit IgG, RT, 2 hours

Tertiary Incubation

Streptavidin-peroxidase / AEC choromagen

Detection

ABC-peroxidase method

Results Summary

It works very specific, no background signal, both stain cajal cells and gastrointestinal stromal tumor in human tissues. I've tried on canine tissues but get no signal. Antibody is very expensive and works on low dilution.

DOI or PMID #

N/A

Additional Notes

Gill's hematoxylin as a background staining.

Image Gallery

Summary

The Good

Specific and sharp.

The Bad

Expensive and low dilution.

The Bottom Line

For human tissues I can say it is great. But expensive and working on low dilution.

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