Abcam
Recombinant Anti-Iba1 antibody
ab178847
EPR16589
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Iba1 was detected in formalin fixed paraffin embedded sections of mouse brain using Rat Anti-Mouse Iba1 Protein A purified Monoclonal Antibody (Catalog # ab178847) at a dilution of 1:1000 overnight at 4 °C. Hoechst 33342 at 1:5000 used for nuclear stain during secondary incubation. The Iba1 staining denoted microglia and potential monocytes and/or macrophages in brain sections proximal to brain microvasculature. This image was taken on a Leica DMi8 epifluorescence microscope with HC PL FLUOTAR 20x/0.55 DRY.
Immunohistochemistry
Mouse, brainstem sagittal section
1:1000, Over-night, 4°C
5% NGS
Chicken Anti-Rabbit IgG H&L (Alexa Fluor® 647) 150 minutes, Room-Temperature + Hoechst 1:5000
N/A
Epifluorescence Microscopy
To identify Iba1+ cells in the brains of aged mice. The selected representative image shows the full acquired view at 20x in the brainstem of aged mice.
Reliable and highly specified signal to microglia.
Expensive
Expensive but good signal even at very dilute concentrations.