Great Nuclear Proteins Antibody from CST

Cardiovascular Biology
OMRF
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Histone H3 Antibody

Catalog Number:

14269S

Clone Number:

N/A

Histones, the main protein components of chromatin, are small basic proteins highly conserved in eukaryotes. Histone H3 is a highly express in nuclear protein and dynamic chromatin modification that impinges on the epigenetic landscape of eukaryotic cells in several ways. H3 phosphorylation contributes to the general condensation of chromatin during mitosis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse tissue liver and Heart

Primary Incubation

1:1000 dilution overnight incubation

Blocking Agent

4% Skimmed milk

Secondary Incubation

1:10000 4% milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We used our experiment for membrane and nuclear protein separation in tgfbeta induced time dependent increased Chemoattractant. Histone H3 is specifically phosphorylated during both mitosis and meiosis. this is specific for nuclear protein.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great

The Bad

None

The Bottom Line

Perfect nuclear antibody.

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