Good Transfer Buffer from Bio-Rad

OMRF
Cardiovascular Biology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

10X Glycine Transfer buffer

Catalog Number:

1610771

In our lab, we usually used 10X Glycine Transfer buffer from Bio-Rad. This buffer makes 1X and is ready easily, and the western blot transfers the protein perfectly and gets good results.

Experimental Design and Results Summary

Application

Western blot transfer

Starting Material

N/A

Tips

High-quality transfer buffer.

Results Summary

In general, proteins can be successfully transferred by applying 25-30 V in a semi dry transfer system. Some proteins requiring improved transfer efficiency onto the membrane include large proteins exceeding 100 kDa or very hydrophobic proteins.

DOI or PMID #

N/A

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Summary

The Good

Great

The Bad

None

The Bottom Line

This 10X glycine good transfer buffer.

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