Excellent Mitochondrial Superoxide Indicators from Thermo Fisher

April 27, 2023

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HMRI
Nanomedicine
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Company:

Thermo Fisher Scientific

Product Name:

MitoSOX™ Mitochondrial Superoxide Indicators, for live-cell imaging

Catalog Number:

M36008

I used this product for staining of mitochondria related ROS on A549, MRC5 and SHSY5Y cells. I used a dilution factor of 1:1000 in serum free medium for 10 minutes in the incubator. Cells were then fixed and imaged. Excellent staining. Recommended.

Experimental Design and Results Summary

Application

Immunofluorescence imaging

Starting Material

N/A

Tips

Has to be used on live cells

Results Summary

I used this product for staining of mitochondria related ROS on A549, MRC5 and SHSY5Y cells. I used a dilution factor of 1:1000 in serum free medium for 10 minutes in the incubator. Cells were then fixed and imaged. Excellent staining. Recommended.

DOI or PMID #

N/A

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Summary

The Good

Specificity and absence of background fluorescence.

The Bad

N/A

The Bottom Line

I used this product for staining of mitochondria related ROS on A549, MRC5 and SHSY5Y cells. I used a dilution factor of 1:1000 in serum free medium for 10 minutes in the incubator. Cells were then fixed and imaged. Excellent staining. Recommended.

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