Great Control for PLA and WB

April 18, 2023

BlueskyReddit
Neurosurgery
Penn State College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Actin Antibody

Catalog Number:

ab197345

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: Actin Antibodies

Used for proximity ligation assay and works great on HEK 293 cells at 1:200 dilution. Used in combination with vinculin to show positive control interactions Works for WB using human and mouse samples at 1:1000.

Experimental Design and Results Summary

Applications

Proximity Ligation Assay

Sample

HEK 293 cells

Primary Incubation

1:200

Blocking Agent

DuoLink Blocking buffer

Secondary Incubation

DuoLink Probes

Tertiary Incubation

N/A

Detection

DuoLink Reagent Red

Results Summary

Worked great as a positive control in combination with vinculin for proximity ligation assay. I used it to optimize the method. It is also a great antibody for western blot loading controls.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great control for all applications.

The Bad

Nothing

The Bottom Line

Would highly recommend.

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