Thermo Fisher Scientific (Invitrogen)
Caveolin 1 Monoclonal Antibody
MA5-16329
SP43
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Expression of Caveolin-1 on brain endothelial cells increases during inflammation and is associated with increased BBB permeability. We analyzed Cav-1 expression on blood vessels in the brainstem as a result of viral respiratory infection in mice.
Immunofluorescence
Mouse brainstem
1:500 in PBS + 0.01% Triton-X100, overnight at 4C
PBS + 0.01% Triton-X100 + 10% BSA
Goat anti-rabbit AF647 1:500 in PBS+0.1% Triton-X100, 2hr at 25C
N/A
Epifluorescence microscopy
Cav-1 signal was increased at brainstem blood vessels in mice infected with respiratory virus. However, there was significant background tissue staining of Cav-1 with this antibody.
Bright signal.
A lot of background staining.
This antibody works very well to stain tissue for immunofluorescence. However, it appears to have a lot of non-specific staining in myelinated tissue.