Specific CD47 Antibody for Western Blot

osaka metropolitan university

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Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Purified anti-mouse CD47 Antibody

Catalog Number:

127501

Clone Number:

miap301 

Info:Supplier Page View Product Specs

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Western blot was performed using cell lysates of mouse Lewis lung carcinoma, and only specie-specific bands were obtained in the desired size.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Lewis lung carcinoma cell lysate

Primary Incubation

1:1000, 4℃, O/N

Blocking Agent

5% skim milk

Secondary Incubation

anti-rat IgG-HRP

Tertiary Incubation

1:10000

Detection

Immobilon Western Chemiluminescent HRP Substrate (Merk Millipore)

Results Summary

Western blot was performed using cell lysates of mouse Lewis lung carcinoma, and only specie-specific bands were obtained in the desired size (about 50 kDa).

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Specificity is good.

The Bad

N/A

The Bottom Line

We were very grateful for the small quantity of scale products (50 ug).

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