Simple and Highly Sensitive PCR-Based Test to Detect Mycoplasma

April 18, 2023

BlueskyReddit
MD Anderson Cancer Center - University of Texas
Cancer System Imaging (Diagnostic Imaging Division)
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

ATCC

Product Name:

Universal Mycoplasma Detection Kit

Catalog Number:

30-1012K

In our lab we bought the ATCC kit to run a comparison with another cheaper PCR-based kit we are currently using. The kit is simple and quick, and the positivity for mycoplasma is easily detected with an electrophoretic run on a 3% agarose gel (size of the positive band goes from 434 to 468 bp). The ATCC kit resulted to be more reliable in terms of true positives detected compared to the other kit.

Experimental Design and Results Summary

Application

Mycoplasma detection

Starting Material

Cells culture

Tips

lysis necessary before running the PCR, loading buffer necessary before running the electrophoretic gel.

Results Summary

In our lab we bought the ATCC kit to run a comparison with another cheaper PCR-based kit we are currently using. The kit is simple and quick, and the positivity for mycoplasma is easily detected with an electrophoretic run on a 3% agarose gel (size of the positive band goes from 434 to 468 bp). The ATCC kit resulted to be more reliable in terms of true positives detected compared to the other kit.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Simple, quick, no false positive.

The Bad

Expensive

The Bottom Line

Simple and highly sensitive PCR-based test to detect mycoplasma.

Join the discussion