Peprotech
Human TNF-α Mini TMB ELISA Development Kit
900-TM25
Info:Supplier Page
This comes in two formats: 2 plates or 10 plates. The standard procedure for TMB ELISA provided sensitive and specific results.
ELISA
Tissue culture supernatants from THP-1 cells
Used BSA in blocking and assay diluent
Robust amount of TNF-alpha was detected with overnight stimulation with zymosan (10ug/ml). Little to no background with unstimulated cells.
N/A
Affordable and works well.
Linear range was between 100pg/ml to 1000pg/ml.
Works well and is inexpensive.