Excellent PFKFB3 Antibody from Cell Signaling Technology

April 17, 2023

Nanomedicine
HMRI
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

PFKFB3 antibody, Rabbit

Catalog Number:

13123

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: PFKFB3 Antibodies

I used this product for evaluating PFKFB3 expression on human neuronal cells by WB. The antibody detects one band at 60 kDa of molecular weight. The antibody has been diluted 1:1000 in 5% BSA in TBST 1X and kept overnight. Secondary antibody diluted 1:5000 was incubated for 2 hours.

Experimental Design and Results Summary

Applications

Western Blot

Sample

SH-SY5Y

Primary Incubation

1:1000 overnight

Blocking Agent

5% BSA in TBST 1x

Secondary Incubation

1:5000 2 hours

Tertiary Incubation

N/A

Detection

Western Blot

Results Summary

I used this product for evaluating PFKFB3 expression on human neuronal cells by WB. The antibody detects one band at 60 kDa of molecular weight. The antibody has been diluted 1:1000 in 5% BSA in TBST 1X and kept overnight. Secondary antibody diluted 1:5000 was incubated for 2 hours.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very strong and clean bands.

The Bad

N/A

The Bottom Line

I used this product for evaluating PFKFB3 expression on human neuronal cells by WB. The antibody detects one band at 60 kDa of molecular weight. The antibody has been diluted 1:1000 in 5% BSA in TBST 1X and kept overnight. Secondary antibody diluted 1:5000 was incubated for 2 hours.

Share your experience with other scientists. Write a Review! »

Join the discussion