Just OK Antibody for Flow Cytometry

April 28, 2023

BlueskyReddit
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti-mouse CD275 (ICOSL)

Catalog Number:

107405

Clone Number:

HK5.3

Info:Supplier Page View Product Specs

Browse Similar Products: CD275 Antibodies

I use this antibody in my regular panel to detect ICOSL positivity in murine lymphocyte populations by flow cytometry. It is still pretty weak, even in PE, and has poor separation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine lymphocytes

Primary Incubation

1:200, 4C for 20 min

Blocking Agent

Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This product helps me determine ICOSL positivity in murine lymphocyte populations via flow cytometry.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

None

The Bad

Poor signal and hard to gate positive from negative subpopulations.

The Bottom Line

This antibody is fairly difficult to use for flow cytometry of murine lymphocyte populations.

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