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Antimouse CD274 (PD-L1) in BV421
124315
10F.9G2
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I use this antibody to mark PD-L1 positivity in murine secondary lymphoid organs via spectral flow cytometry. Although it gives a smear from positive to negative, the signal is pretty strong.
Flow Cytometry
Murine lymphocytes
1:300, 4C for 20 min
Fc block
N/A
Flow cytometry
This antibody is helpful to distinguish PD-L1 positive versus negative populations in murine lymphocytes via flow cytometry.
Strong signal.
Get a smear of positive to negative signal, not good separation.
Overall this antibody is OK but not the best for murine lymphocytes via flow cytometry.