Good Siglec F Antibody for Flow Cytometry

April 27, 2023

UCSF
Graduate Student

Overall

Quality of Results

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Company:

Thermo Fisher Scientific

Product Name:

Anti-mouse SiglecF antibody in eFluor660

Catalog Number:

50-1702-82

Clone Number:

1RNM44N

Info:Supplier Page View Product Specs

Browse Similar Products: SIGLECF Antibodies

I use this antibody to identify eosinophils in murine lymphoid tissue. I usually gate these out first as eosinophils are auto fluorescent by flow cytometry and it works well and has good separation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine lymphocytes

Primary Incubation

1:1000, 4C for 20 min

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

I use this antibody to gate out eosinophils by flow cytometry in murine lymphoid tissue. It has good signal and separation.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good signal and separation.

The Bad

None

The Bottom Line

I recommend this antibody for flow cytometry staining of murine secondary lymphoid organs.

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