Usable CD3e Antibody but Not Great Separation

April 20, 2023

BlueskyReddit
UCSF
Graduate Student

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Company:

BD Biosciences

Product Name:

CD3e in BUV395

Catalog Number:

563565

Clone Number:

145-2C11

Info:Supplier Page 

Browse Similar Products: CD3E Antibodies

I use this antibody in my regular panel for mouse lymphocytes. I don't get super great separation between CD3+ and CD3- populations, but it is good enough to use.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymphocytes

Primary Incubation

1:300, 20 min @ 4C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

I use this antibody in my regular panel to detect murine lymphocytes. The separation is not great but good enough to use.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good signal.

The Bad

Not great separation.

The Bottom Line

Usable to detect CD3- and CD3+ murine lymphocyte populations.

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