Good PE/Cy5 CD197 Antibody for Flow Cytometry

April 11, 2023

BlueskyReddit
UCSF
Graduate Student

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Company:

BioLegend

Product Name:

PE/Cy5 CD197 (CCR7)

Catalog Number:

120114

Clone Number:

4B12

Info:Supplier Page View Product Specs

Browse Similar Products: CD197 Antibodies

I use this antibody to mark migratory dendritic cells in the mouse lymph node. It works well when stained for 30 minutes at 37C, but not as well at 4C.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse myeloid cells from lymph node

Primary Incubation

1:200, 30 min @ 37C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

This antibody, when stained at 37C, gives good separation of CCR7+ and CCR7- myeloid cells. It is my go to antibody to stain mouse lymph nodes.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good separation.

The Bad

Must stain at 37C.

The Bottom Line

Recommend this antibody for staining mouse immune cells.

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