Easy-to-Use Stripping Buffer from Serva

April 05, 2023

BlueskyReddit
Robert Bosch Gesellschaft für medizinische Forschung mbH
IKP/RBCT
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Serva

Product Name:

BlueClear SB for antibody stripping

Catalog Number:

42599.01

The Serva BlueClear SB is a easy-to-handle mild stripping buffer which we regularly use in our lab to strip antibodies off Western Blot Nitrocellulose membranes. It can be re-used multiple times, stripping time might vary between antibodies.

Experimental Design and Results Summary

Application

Western Blot

Starting Material

Nitrocellulose membrane loaded with primary/secondary antibodies for Chemiluminescence detection (HRP-coupled).

Tips

Incubation 30 min at RT, can be extended for larger amounts of antibodies to be stripped off.

Results Summary

After stripping, membranes can be loaded with a new round of antibodies. Usually the "old" signal is completely gone after stripping - sometimes some weaker signal remains.

DOI or PMID #

N/A

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Summary

The Good

Easy-to-use, efficient stripping.

The Bad

Stripping time optimization needed.

The Bottom Line

Although stripping time is not too short and has to be optimized for some antibodies, the Serva BlueClearSB is a easy-to-handle stripping buffer for your daily use in Western Blotting.

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