Novus Biologicals' PDGF-B Antibody Works Well

March 30, 2023

BlueskyReddit
Pediatrics
Medical College of Wisconsin
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

Rabbit polyclonal PDGF-B antibody

Catalog Number:

NBP1-58279

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: PDGF-b Antibodies

The detection level of PDGF-B antibody in the cell lysate of primary human brain microvascular endothelial cells were good.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysate

Primary Incubation

Antibody dilution of 1:500, overnight incubation at -4C.

Blocking Agent

5%BSA

Secondary Incubation

1:1000. 1 hr incubation in RT

Tertiary Incubation

NA

Detection

Pierce ECL western blotting substrate.

Results Summary

The antibody works well and detects the level of PDGF-B present in the cell lysate at 27 kDa at the dilution of 1:500.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Detects well

The Bad

N/A

The Bottom Line

Antibody signal was accurate, would definitely recommend it.

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