Very Good CD45 Antibody for Mouse

March 27, 2023

BlueskyReddit
Surgery
UCSF
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 605™ anti-mouse CD45 Antibody

Catalog Number:

103155

Clone Number:

30-F11

Info:Supplier Page View Product Specs

Browse Similar Products: CD45 Antibodies

Our lab is interested in understanding mechanism of peripheral tolerance. We are also interested in characterizing the immune populations in secondary lymphoid organs under different knockout condition.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph nodes

Primary Incubation

1:500 at 4deg for 30min

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Aria Fusion

Results Summary

This CD45 antibody is able to distinguish CD45+ cells from non-immune cells from lymph node suspension that has been digested. Easy to use and stain with little to no optimization needed.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very easy to use.

The Bad

N/A

The Bottom Line

Recommended for detection of CD45 even though BV605 is not a bright channel.

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