Great GFAP Antibody for IF

March 20, 2023

BlueskyReddit
Anatomy and Cell Biology
University of Illinois at Chicago
Graduate Student

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Company:

MilliporeSigma

Product Name:

Anti-GFAP antibody

Catalog Number:

HPA056030-100UL

Clone Number:

Polyclonal

Info:Supplier Page 

Browse Similar Products: GFAP Antibodies

Astrocyte activation in the olfactory bulb of mice infected with respiratory virus is analyzed by GFAP staining of brain sections.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain histology sections

Primary Incubation

1:100 in PBS+0.1% Triton-X100 overnight incubation at 4C

Blocking Agent

PBS+0.1% Triton-X100 + 10% BSA

Secondary Incubation

Mouse anti-rabbit IgG AF488 1:500 in PBS+0.1% Triton-X100 2hr at 25C

Tertiary Incubation

N/A

Detection

Epifluorescence microscopy

Results Summary

Enhanced GFAP signal in olfactory bulb of infected mice indicates that respiratory infection results in astrocyte activation and neuroinflammation.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Bright signal.

The Bad

None

The Bottom Line

This antibody works very well for staining mouse tissue for immunofluorescence. Highly recommend.

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