Highly Sensitive Anti-Flag Antibody from BioLegend

March 24, 2023

CIV
Dana-Farber Cancer Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-DYKDDDDK Tag Antibody

Catalog Number:

637309

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: FLAG Antibodies

We use this product to detect proteins with DYKDDDK tag. It is very sensitive. Usually dilution of 1:500 is enough. If you wash the sample properly, there is no background signal.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

1:500, 20 minutes, 4 degrees

Blocking Agent

BSA

Secondary Incubation

1:500, 20 minutes, 4 degrees

Tertiary Incubation

1:500, 20 minutes, 4 degrees

Detection

Flow cytometry

Results Summary

This product can help to evaluate the quality of protein (binding affinity) and also to detect cells with the marker of interest using protein with Flag tag and use this as secondary.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Sensitive

The Bad

None

The Bottom Line

It is affordable and highly sensitive. I recommend this product.

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