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PerCP/Cyanine5.5 anti-human CD14 Antibody
325621
HCD14
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Monocytes represent around 20% of the total peripheral blood mononuclear cells (PBMCs) and they specifically express a subset of receptors, among which CD14. The During our experiments, we have the need of identify this cells both among whole PBMCs or after purification.
Flow Cytometry
Human PBMCs
1:100 per 200-500k cells in 100ul, at 4°C for 20 mins
PBS + FCS 2%
None
BD FACSCanto
Very nice staining, this antibody allowed us to stain and sort monocytes from total human PBMC and to evaluate the purity of the population after a positive, magnetic bead based, selection.
N/A
Good staining.
Good antibody for surface staining of human monocytes, very specific.