Agilent (Dako)
anti-Ki67
M7249
MIB-1
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During our study, we wanted to evaluate the proliferation state of different cell population during kidney development. We used Ki67, in combination with cell specific markers to quantify the cells in active proliferation in our mouse model of congenital malformation of the kidney.
Immunofluorescence
Paraffine embedded embryonic mouse kidneys
1:500 in blocking solution, 1 hour at RT
PBS + donkey normal serum 10%
Flourescence coupled secondary antibody, 1:500 for 1 hour at RT
None
Fluorescence/confocal microscopy
This antibody raised in rat has very strong and specific signal in fixed-paraffin embedded renal samples from mouse embryos at different ages. It can be easily multiplexed with antibodies against cell specific population for an overall estimation of the proliferation state in the whole tissue.
N/A
Very specific staining.
Very useful and reliable antibody from DAKO, but sometimes it might be difficult to purchase directly from the seller according to your region.