Abcam
Anti human Cytokeratin 18
ab52948
Rabbit polyclonal
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We studied the zonation of the liver. The KRT18 is expressed in the whole liver (hepatocytes) with an increase in the epithelial structure surrounding the bile ducts. The expression level of this protein allowed us to follow the development of this tissue in mouse models.
Immunofluorescence
Mouse liver
1:500 in blocking solution, 1 hour at RT
PBS + donkey normal serum 10%
Fluorescence coupled secondary antibody, 1:500 for 1 hour at RT
None
Fluorescence/confocal microscopy
Very strong and specific staining of the liver tissues. This antibody raised against human epitopes proved very efficient and specific also when staining paraffin embedded mouse tissues. The epitope supports well the buffer citrate (pH8 or pH6) antigen retrieval method
N/A
It can be used also for fresh or OCT embedded tissues.
Specific and strong signal
Good antibody to be used in both human or mouse fixed/paraffine embedded tissues.