Excellent Column to Purify/Concentrate DNA

University of Cote d'Azur (UCA)
LP2M
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

New England Biolabs

Product Name:

Monarch PCR and DNA cleanup kit (5ug)

Catalog Number:

T1030L

Biomolecular/cloning protocols often require to purify or concentrate DNA coming from PCR products or digested plasmid. This columns have the advantage, compare to other supplier, to elute in very small volumes (down to 6ul) very useful when dealing with small amount.

Experimental Design and Results Summary

Application

Molecular biology

Starting Material

PCR products or plasmids

Tips

Max 5ug of DNA

Results Summary

These columns allowed us to purify and further concentrate PCR products or digested plasmids. Very good recovery yield, comparable with other main suppliers.

DOI or PMID #

N/A

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Summary

The Good

Small elution volume.

The Bad

N/A

The Bottom Line

Very useful when dealing with small amount of DNA (plasmid or PCR amplicons) to avoid large volumes of elution buffer.

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