Time Course Effect of Chemoattractant on PDE4D Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Invitrogen

Product Name:

PDE4D Antibody

Catalog Number:

PA5-18459

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: PDE4D Antibodies

Phosphodiesterase 4D is one of the member of the PDE cyclic nucleotides. PDE4D consists of a single chain of amino acids with no predicted transmembrane domains. Protein kinase C is known to regulate by PDE4D and also by cAMP in vascular smooth muscle cells. PDE4D is essential to have equilibrium in relaxing and contracting cues to airway smooth muscle. Here we checked the time course effect of PDE4D expression by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 prepared in 1x TBST

Blocking Agent

5% Skim milk

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We performed western blot and detect, analyzed the PDE4D expression. We found that there is a a time dependent increase of PDE4D expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

This is a antibody and works great for western blot.

The Bad

None

The Bottom Line

Works great for western blot and very specific. Highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion