Recommended Perforin (E9F7N) Rabbit mAb for Western Blot

February 22, 2023

BlueskyReddit
Houston Methodist Research Institute

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Company:

Cell Signaling Technology

Product Name:

Perforin (E9F7N) Rabbit mAb

Catalog Number:

44865S

Clone Number:

N/A

I have incubated the primary antibody overnight at 4°C with 5% BSA TBST. The secondary antibody was diluted 1:5000 and the membrane was incubated at room temperature for 3 hours. I got great results.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Fibroblast

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:5000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

I have incubated the primary antibody overnight at 4°C with 5% BSA TBST. The secondary antibody was diluted 1:5000 and the membrane was incubated at room temperature for 3 hours. I got great results.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

Really recommended.

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