Good Plasmid Miniprep Kit from New England Biolabs

University of Cote d'Azur (UCA)
LP2M
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

New England Biolabs

Product Name:

Monarch® Plasmid Miniprep Kit

Catalog Number:

T1010L

Working on cell lines, we need to produce high quality plasmids with rapidity. The purification procedure is as fast as the other suppliers, but with the proprietary funnel-shaped tubes, this kit allows to concentrate much more the plasmids at the elution step.

Experimental Design and Results Summary

Application

Plasmid purification

Starting Material

Bacteria culture

Tips

Bacteria should not be too confluent.

Results Summary

Very easy to use kit. Different dyes in the solutions allow to follow the lyse/neutralisation steps very efficiently. Small elution volume are very handy when dealing with low yield of plasmids.

DOI or PMID #

N/A

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Summary

The Good

Small elution volume.

The Bad

we often observe contaminant still present in the elution.

The Bottom Line

Good plasmid purification kit for minipreps. It performs has well has the the other suppliers and it has the advantage of concentrating more the eluted plasmids.

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