Invitrogen
IgA Monoclonal Antibody, FITC
11-4204-82
mA-6E1
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we were looking at IgA+ PCs in the spleen of a WT mouse.
Flow Cytometry
C57BL/6 spleen
surface staining with IgA FITC (1:100), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer BD CANTO
We could detect IgA expressing plasma cells. Around 20% of the plasma cells (CD138hi) in the spleen.The signal is not very strong, but a populations of IgA+ cells can clearly be identified. To improve the staining the antibody can be used at a 1/50 dilution.
The anti-IgA APC (same company) works better.
It works.
Weak signal.
The signal is weak, so if you can is better to use anti-IgA APC (same company).