Invitrogen
IgA Monoclonal Antibody, APC, eBioscience
17-4204-82
mA-6E1
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Browse Similar Products: IgA Flow Cytometry Antibodies
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at quantify the frequency of IgA+ PCs in the spleen of a WT mouse.
Flow Cytometry
C57BL/6 spleen
Surface staining with IgA APC (1:100), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer with a red laser.
As it can be observed in the figure, we found that around 25% of the PCs (CD138hi) in the spleen express IgA+ at the surface.
Reliable with good signal.
Expensive.
It is expensive, but a reliable option.