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APC/Cyanine7 anti-mouse CD3 Antibody
100221
17A2
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody-secreting cells (ASC) in health and disease. Although, we are usually not staining T cells, or just staining them for exclusion, in this case we stained CD3 T cells in spleen and mLN of WT mice.
Flow Cytometry
Spleen and mLN of C57BL/6
Surface staining with CD3 APC-Cy7 (1:200), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer with a red laser (in this case a CANTO)
We stained CD3 T cells in spleen and mLN, as well as CD19 B cells. First, we gated in lymphocytes and singlets. Regarding frequencies of T cells, as expected the frequency of CD3 cells in higher in the LN than in the spleen.
There is a clear signal.
It is not very bright.
It has a reasonable prize and it works, but it could be brighter.