Highly Recommended Dispase II for Tissue Digestion

UCSF
BMS
Graduate Student

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Company:

MilliporeSigma

Product Name:

Dispase II

Catalog Number:

D4693

The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the immune system– the mammalian defense to homeostatic alteration. Ketogenic diets (KDs) are extremely high in fat and very low in carbohydrates and have been shown to dramatically rewire whole-body metabolism. The KD-induced metabolic adaptation improves many physiological processes including aging, fatty acid utilization, and glucose homeostasis. However, the effects of KDs on host immunity remain less explored1–3. Thus, the overall objective of my PhD studies will be to elucidate how KDs affect the immune response in adaptive and maladaptive contexts.

Experimental Design and Results Summary

Application

Tissue digestion

Starting Material

Mouse tissue

Tips

2U/mL was added for mouse skin digestion.

Results Summary

Dispase II (stock 0.5 U/mg) (#D4693-1G. Sigma): 2U/mL was used for mouse skin digestion. The digestion successfully yielded viable single-cell immune populations.

DOI or PMID #

N/A

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Summary

The Good

Reliable results.

The Bad

N/A

The Bottom Line

Highly recommended for mouse skin digestion.

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