Great Staining Buffer Set for Intracellular Staining for Flow Detection

UCSF
BMS
Graduate Student

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Company:

Thermo Fisher Scientific (eBioscience™)

Product Name:

Foxp3 / Transcription Factor Staining Buffer Set

Catalog Number:

00-5523-00

The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the immune system– the mammalian defense to homeostatic alteration. Ketogenic diets (KDs) are extremely high in fat and very low in carbohydrates and have been shown to dramatically rewire whole-body metabolism. The KD-induced metabolic adaptation improves many physiological processes including aging, fatty acid utilization, and glucose homeostasis. However, the effects of KDs on host immunity remain less explored1–3. Thus, the overall objective of my PhD studies will be to elucidate how KDs affect the immune response in adaptive and maladaptive contexts.

Experimental Design and Results Summary

Application

Intracellular staining and transcription factor staining.

Starting Material

N/A

Tips

Fix/perm 30min to overnight is acceptable with mouse samples.

Results Summary

This staining kit works well with Foxp3 TF staining as well as the majority of intracellular cytokine staining, such as IL-4, IL-13, IL-17.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Reliable results.

The Bad

N/A

The Bottom Line

Good kit for Intracellular staining and transcription factor staining.

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