Ubiquitin Antibody Did Not Work for Me

February 06, 2023

Neurosurgery
Penn State College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

Ubiquitin antibody

Catalog Number:

NB300-130

Clone Number:

N/A

I tried 4 different Ub antibodies (two of which have been published on in my field and protein of interest) and this is one that did not work. A friend told me that her lab uses this one all the time, but not for me I guess.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK 293 cells

Primary Incubation

1:1000 overnight BSA

Blocking Agent

BSA

Secondary Incubation

1 hr, milk

Tertiary Incubation

N/A

Detection

ECL

Results Summary

I tried to use this to confirm the ubiquitination of ferroportin by hepcidin. I did not with this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Nothing.

The Bad

Did not work for me.

The Bottom Line

Would not recommend.

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