Good CD14 Antibody for Human Monocytes

AG 61
Leibniz Institute für Virologie
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD14 anti-human BUV737

Catalog Number:

612763

Clone Number:

M5E2

The antibody is working perfectly on human PBMCs to distinguish classical and intermediate monocytes. Good brightness and reliability

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1µl/sample, 20 minutes,RT

Blocking Agent

NHS

Secondary Incubation

No secondary incubation needed

Tertiary Incubation

No tertiary incubation needed

Detection

BUV737

Results Summary

Good brightness with a concentration of 1µl/ sample. It´s working well on human PBMCs and the brightness is maintained also after fixation.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable and good brightness.

The Bad

Expensive.

The Bottom Line

Good product for flow cytometry. It has a good brightness and reliability between samples. Highly recommended.

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