Cell Trace Violet to Quantify Proliferation

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Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

Cell Trace Violet

Catalog Number:

C34557 A

I work on immune responses between target cells such as HEK cells or B-EBV cells. To quantify this immune response, I use this product on PBMCs to see if they proliferate more depending on the target cells used during co-culture.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

N/A

Tips

Mix this product in DMSO just before using it

Results Summary

This product enabled me to give a proof that some cells activate more CD8+ cells than other cells depending on HLA expression.

DOI or PMID #

N/A

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Summary

The Good

Efficient

The Bad

Quite hard to to have proper result first time using it.

The Bottom Line

Even if my very first experiments did not give clear results, I can now have precise data showing differences of CD8+ cells proliferation depending on target cells during co-culture. I recommend this product.

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