Valid Option to Stain for Ly108

December 22, 2022

BlueskyReddit
Charite Strasse 1
Deutsches Rheuma-Forschungszentrum
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BV711 Mouse Anti-Mouse Ly-108

Catalog Number:

740823

Clone Number:

13G3

Info:Supplier Page 

Browse Similar Products: Ly108 Flow Cytometry Antibodies

In different healthy and pathological contexts, CD4 and CD8 T cells differentiate into a range of specialized effector and regulatory subsets. In mouse models of aging and autoimmune diseases we study the effector and memory phenotype of CD4 and CD8 T cells. We identified different subsets of naïve and activated CD4 and CD8 T cells with an anti-Ly108 BV711 antibody in 24-month-old C57BL/6 mouse.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes of wild-type 24-month-old C57Bl/6 mouse

Primary Incubation

Washed twice in PBS and incubated with L/D fixable aqua dye 20 min at 4°C, then washed twice in PBS.

Blocking Agent

2.4G2

Secondary Incubation

Surface staining with anti-CD3 BUV805, anti-CD4 BUV495, anti-CD8 FITC, anti-CD62L BV605, anti-Ly108-BV711 1/150 etc., in Cell Staining Buffer (Biolegend) incubated 30 min at 4°C.

Tertiary Incubation

N/A

Detection

Violet laser on Symphony cytometer

Results Summary

Different populations of CD4 and CD8 T cells are identified: CD62L+Ly108- naive cells, and CD62L-Ly108+ activated cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody is specific and reliable.

The Bad

Not as bright as other fluorochromes, careful compensations controls should be done.

The Bottom Line

Usable, and good option to use the BV711 channel. Some caution for the compensations should be taken, in particular in PerCP Cy5.5 channel.

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