Nice Calcein AM, Cell-Permeant Dye

Houston Methodist Research Institute
Nanomedicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen™

Product Name:

Calcein, AM

Catalog Number:

C3100MP

We used this Calcein AM to stain the live cell. Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent calcein AM is converted to a green-fluorescent. We tested in several different cells. It worked great.

Experimental Design and Results Summary

Application

Determine cell viability

Starting Material

N/A

Tips

Store in -20 and protect from light.

Results Summary

We used both flow cytometry and confocal to observe the cells incubated with Calcein AM. It worked good and we were able to see the live cells.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Easy to use and reliable.

The Bad

Expensive

The Bottom Line

Calein AM has great signal, recommended!

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