Fujifilm Wako
Anti Iba1, Rabbit antibody
019-19741
N/A
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I have used this antibody to study microglia morphology within brain from an animal model of inflammatory pain. It has worked very well in mouse sample.
Immunofluorescence
Mouse spinal cord
Incubation was done with Ab diluted to 1:500 for 16h at 4ºC
BSA 4% in PBS
Incubation was done with 2nd Ab diluted to 1:1,000 for 3h at room temperature
None
Fluorescence (AF 488)
In all cases reactivity is quite good at 1:500 dilution when neural inflammation is strong. This protein increases expression under these circumstance so the detection is facilitated in experimental models of inflammation. I have captured this image in my confocal to study morphology of microglia process (please see my paper for details).
31354431
Very good reactivity.
I have experienced no issues with this product.
Great antibody to study microglia morphology.