Excellent Western Blot Stripping Buffer

Houston Methodist Research Institute
Nanomedicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific™

Product Name:

Restore™ Western Blot Stripping Buffer

Catalog Number:

21059

We have been using this buffer to strip the bands for Western Blot. This buffer works great and can safely and effectively remove primary and secondary antibodies from both nitrocellulose and PVDF membranes.

Experimental Design and Results Summary

Application

Western Blot

Starting Material

N/A

Tips

This buffer can be reused for at lease 3 times if you store in -4C

Results Summary

Using this stripping buffer, primary and secondary antibodies from immunoblots were cleaned and efficiently removed without removing or damaging the immobilized antigen.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Reliable. Multiple stripping will not damage the antigen.

The Bad

N/A

The Bottom Line

A great buffer for Western Blot stripping, Highly recommended.

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