Western Blot on Mouse Lung Tissue

December 13, 2022

BlueskyReddit
Northbridge Biomedical Research Institute

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Company:

Abcam

Product Name:

Anti-TGF beta 1 antibody

Catalog Number:

ab92486

Clone Number:

N/A

I used this antibody on mouse lung tissue. Even if it's recommended to use for human cells I was able to detect the protein. The protein expression was detected at 25kDa.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lung tissue

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:5000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

I used this antibody on mouse lung tissue. Even if it's recommended to use for human cells I was able to detect the protein. The protein expression was detected at 25kDa.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

I recommended event if I used for mouse instead of human.

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