Excellent CD57 Antibody

December 13, 2022

Immunology
UiO
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

Brilliant Violet 605(TM) anti-human CD57 from BioLegend

Catalog Number:

393303

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: CD57 Antibodies

We are an immunotherapy focused group with an interest in CD8 T-cells and their TCRs. For our experimental purposes, we routinely isolate naïve T-cells from healthy blood donors. This antibody is used in our panel to determine the naive population purity after isolation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Naive T-cells

Primary Incubation

1:100, 15min RT

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

PBMCs were isolated from blood donors and after magnetic isolation of the naïve T-cells (CD57pos), the purity of the sample was measure using a FACs staining panel.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Bright and reliable staining.

The Bad

None

The Bottom Line

This is a great a CD57 antibody, with BV605 providing a bright signal. The PE/APC versions would be recommended if brighter signal is required. The antibody is well priced to begin with and little amounts of the antibody are required.

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