Good CD57 MicroBeads for Magnetic Isolation

UiO
Immunology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD57 Microbeads

Catalog Number:

130-092-073

We are an immunotherapy focused group, with interest in CD8 T-cells and their TCRs. For our experimental purposes, we routinely require naive T-cells from healthy blood donors. We are using this beads to perform magnetic isolation of the naive population, using the Automacs Pro Separator.

Experimental Design and Results Summary

Application

Magnetic Isolation

Starting Material

PBMCS

Tips

If many cells, it's best to split the sample in 2 separate tubes.

Results Summary

PBMCs were isolated from blood donors and naive T-cells were isolated with the help of these beads (alongside CD8 and CD45ro).

DOI or PMID #

N/A

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Summary

The Good

Easy to use, works well.

The Bad

Very expensive and the expiry date is not that long.

The Bottom Line

These beads are performing well and in conjunction with the Auto-Macs the isolation doesn't take that long.

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