Good Kit for Myelinated Cell Removal

December 13, 2022

BlueskyReddit
University of Illinois at Chicago
Anatomy and Cell Biology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Myelin Removal Beads II

Catalog Number:

130-096-731

We used this kit to remove myelinated cells from mouse brain homogenate and enrich for endothelial cells. The protocol was fast and worked well to remove myelinated cells and contaminating myelin proteins.

Experimental Design and Results Summary

Application

Primary cell culture

Starting Material

Mouse brain homogenate

Tips

Incubate cells with beads in 4C fridge, not on ice.

Results Summary

Flow cytometry analysis showed the Myelin Removal Beads removed small, granular cells and debris from our brain tissue prep. Using the kit, we were able to enrich for CD31+ endothelial cells.

DOI or PMID #

N/A

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Summary

The Good

Fast protocol.

The Bad

Requires proprietary columns from manufacturer.

The Bottom Line

This kit works very well to remove myelinated cells from CNS tissue preps when the protocol is followed and the proper columns are used. We have incorporated it into our protocol for brain endothelial cell isolation.

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