Transduction with Less Toxicity

MD Anderson Cancer Center - University of Texas
Cancer System Imaging (Diagnostic Imaging Division)
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Lipofectamine 3000 Reagent

Catalog Number:

L3000075

We use Lipofectamine 3000 reagent to transfect 293 cells for lentivirus production. This reagent is very gentle and allows us to lower the dose and so the toxicity risk when working with your cell line.

Experimental Design and Results Summary

Application

Immortalised cell line Transfection

Starting Material

N/A

Tips

Optimization needed

Results Summary

We use Lipofectamine 3000 reagent to transfect Immortalised cell line - 293 cells - for lentivirus production. This reagent is very gentle and allows us to lower the dose and so the toxicity risk when working with your cell line.

DOI or PMID #

N/A

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Summary

The Good

Less toxicity.

The Bad

N/A

The Bottom Line

Transfection of cells with a lower dose and less toxicity.

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