Excellent Anti-GRP78 BiP Antibody

December 08, 2022

Internal Medicine
Yale University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-GRP78 BiP antibody (ab21685)

Catalog Number:

ab21685

Clone Number:

N/A

Used this antibody to investigate the expression of GRP78 in the human and mice platelets following induction of ER stress.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human platelets

Primary Incubation

1:1500

Blocking Agent

BSA

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminscence

Results Summary

Used this antibody for both immunoblotting and immunofluorescence studies in human and mouse platelet and tissue samples. Easy to use and works as recommended. Helped determine the effect of ER stress on the GRP78 expression.

DOI or PMID #

10.1161/CIRCRESAHA.121.320530

Additional Notes

N/A

Image Gallery

Summary

The Good

Works great with a very strong reproducible signal.

The Bad

In case using the same blot for tripping, stripping needs to be done properly. Can give non specific signal for future experiments otherwise.

The Bottom Line

Excellent antibody with many applications.

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