Good Buffer for Crosslinking Process

Houston Methodist Academic Institute

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

MES, 0.5M buffer solution, pH 5.5

Catalog Number:

J62534.AE

I use MES buffer solution for the crosslinking of proteins onto a collagen-based membrane.I used to prepare my MES buffer, but sometimes the results were not satisfying.With this MES buffer solution ready to use I have solved all the issues and now I can obtain a proper crosslinking every time I do it.

Experimental Design and Results Summary

Application

Crosslinking of protein

Starting Material

MES, 0.5M buffer solution, pH 5.5

Tips

N/A

Results Summary

The ready-to-use MES buffer helped me obtaining proper crosslinking every time I do it. No poor crosslinking results and effective functionalization of the membrane I am developing.

DOI or PMID #

N/A

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Summary

The Good

Easy to use. Standard buffer characteristics.

The Bad

N/A

The Bottom Line

I used to have a poor crosslinking when I prepared my own MES buffer. With this ready-to-use MES buffer solution I solved all the issues related to poor crosslinking.

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