Excellent Antibody for Human HLA-A2 Specific Staining

LP2M
University of Cote d'Azur (UCA)
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-human HLA-A2 Antibody

Catalog Number:

343326

Clone Number:

BB7.2

Info:Supplier Page View Product Specs

Browse Similar Products: HLA A2 Antibodies

HlA-A2 is a class I MHC and it is often involved in allograph rejects after organ transplant. This antibody helped us to specifically identify this HLA subgroup from the others.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

HEK293T and human primary cells

Primary Incubation

1:100 per 200-500k cells in 100ul, at 4°C for 20 mins

Blocking Agent

PBS + FCS 2%

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS Aria II

Results Summary

This antibody allow us to identify and follow the cells (HEK293 cell line or primary human cells) specifically expressing on the surface this MHC class I and how they react to different stimuli.

DOI or PMID #

DOI: 10.1111/tan.12095

Additional Notes

N/A

Image Gallery

Summary

The Good

Extremely specific

The Bad

None

The Bottom Line

Very good antibody for FACS staining. The fluorescence is stable and the BV421 spectrum shows very low leakage in the other channels.

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